Abstract: The main aim of this study was compare lower region muscles flexibility of college students. For the purpose of study a total of 150 students were selected from various Colleges of Udaipur. The selected subjects were divided into three age groups i.e. eighteen, nineteen, and twenty year respectively. Sit and reach test was administered to measure the lower region flexibility. There was a significant difference obtain in the lower region and hamstring muscle between the three age groups (eighteen, nineteen, and twenty year). It can be concluded that the twenty year college students had better lower region flexibility when compared to the other two age group selected for the study.