Abstract: Tinosporacordifolia (commonly known as Guduchi or Giloy) is a medicinal climbing shrub widely used in traditional medicine for its immunomodulatory, hepatoprotective, and antidiabetic effects. The therapeutic potential of the plant is linked to its rich secondary metabolites, particularly alkaloids, glycosides, steroids, and phenolics. In the present study, chemical profiling of T. cordifolia stem extracts was performed to establish a comprehensive phytochemical fingerprint. Qualitative and quantitative assays confirmed the presence of diverse bioactive compounds, while chromatographic techniques highlighted key chemical markers such as berberine, tinosporaside, and cordifolioside. The results validate the pharmacological relevance of T. cordifolia and emphasize the importance of standardized phytochemical profiling for ensuring efficacy and safety of herbal formulations. This study provides a baseline for further pharmacological investigations and supports the integration of T. cordifolia into evidence-based herbal therapeutics.

Keywords: Tinosporacordifolia, phytochemical profiling, alkaloids, glycosides, tinosporaside, herbal medicine, therapeutic potential.